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MTT Assay

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3-(4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay is a colorimetric assay based on the ability of viable cells to change from soluble yellow tetrazolium salt to blue formazan crystals. After treatments, 1000 µl of MTT solution (0,5 mg/ml in PBS) was added to each well and incubated for 4 h. Ten percent of sodium dodecyl sulfate (SDS) was then added for another 12 h to thoroughly dissolve the dark blue crystals. The absorbency at 595 nm was measured by an Elisa Reader (Biorad). Activity of the mitochondrial energy metabolism was shown as cell viability (% of control).

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menebar ilmu pengetahuan

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